RESUMO
Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis (ATCC 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with MB and MG. Samples were individually irradiated by the diode laser at a distance of 1mm for 30, 60, or 120 seconds. Colonyforming units (CFU) were determined for each treatment. PDT for 60 and 120 seconds with MG reduced E. faecalis viability significantly. Similar results were obtained when MB was used as photosensitizer. PDT using MB and MG have antibacterial effect against E. faecalis, showing potential to be used as an adjunctive antimicrobial procedure in endodontic therapy.
Assuntos
Enterococcus faecalis/efeitos dos fármacos , Lasers Semicondutores/uso terapêutico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Carga Bacteriana/efeitos dos fármacos , Humanos , Azul de Metileno/uso terapêutico , Viabilidade Microbiana/efeitos dos fármacos , Irrigantes do Canal Radicular/uso terapêutico , Corantes de Rosanilina/uso terapêutico , Hipoclorito de Sódio/uso terapêutico , Fatores de TempoRESUMO
Photodynamic therapy (PDT) has been proven to be effectivein disinfecting root canals. The aim of this present study wasto evaluate the effects of PDT on the viability of Enterococcusfaecalis using methylene blue (MB) and malachite green(MG) as photosensitizers. Solutions containing E. faecalis(ATCC 29212) were prepared and harvested by centrifugationto obtain cell suspensions, which were mixed with MBand MG. Samples were individually irradiated by the diodelaser at a distance of 1mm for 30, 60, or 120 seconds. Colonyformingunits (CFU) were determined for each treatment.PDT for 60 and 120 seconds with MG reduced E. faecalis viabilitysignificantly. Similar results were obtained when MBwas used as photosensitizer. PDT using MB and MG haveantibacterial effect against E. faecalis, showing potential tobe used as an adjunctive antimicrobial procedure in endodontictherapy...
Assuntos
Humanos , Meios de Cultura , Enterococcus faecalis/isolamento & purificação , Fotoquimioterapia/métodos , Lasers Semicondutores/uso terapêutico , Análise de Variância , Contagem de Colônia Microbiana , Cavidade Pulpar/microbiologia , Irrigantes do Canal Radicular/farmacologiaRESUMO
Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis (ATCC 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with MB and MG. Samples were individually irradiated by the diode laser at a distance of 1mm for 30, 60, or 120 seconds. Colonyforming units (CFU) were determined for each treatment. PDT for 60 and 120 seconds with MG reduced E. faecalis viability significantly. Similar results were obtained when MB was used as photosensitizer. PDT using MB and MG have antibacterial effect against E. faecalis, showing potential to be used as an adjunctive antimicrobial procedure in endodontic therapy.
